Abstract: 　Objectives　To investigate the effect of pediatric trauma life support (PTLS) curriculum on the improvement of rescue skill for pediatric trauma patients. Methods　PTLS course was introduced to train medical staff engaging in pediatric critical trauma care. The course included theoretical and practical part with main focus on enhancement of students’ decision on scene and rescue skill. Results　Compared with pre-training, the students’ post-training written test scores improved significantly, and the rates of qualification and excellence were improved significantly (P<0.01). All students were qualified for the tests of skill stations and simulation scenario. The excellence rates of the tests were as follows: fixation and transportation 42.62%, basic and advanced airway management 81.97%, shock evaluation and management 70.49%, simulation scenario 72.13%. Both pre-training and post-training written test scores in hospital group were significantly higher than those in pre-hospital group (P<0.01). Conclusions　After PTLS courses, the knowledge, practice and integrated life-saving skills of students are greatly improved and teamwork is strengthened.